Simple, Sensitive Zymogram Technique for Detection of Xylanase Activity in Polyacrylamide Gels
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چکیده
منابع مشابه
Demonstration of rhodanese activity in polyacrylamide gels.
Rhodanese activity from crude extracts of Thiobacillus sp. strain IV-85 was demonstrated in polyacrylamide gels after incubation in the reaction mixture by staining with dichloroindophenol in the presence of methylphenazonium methosulfate. The sensitivity of the staining system was found to be 8 x 10 moles of sulfite.
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A method for the detection and quantitation of unlabeled nucleic acids in polyacrylamide gels is presented. The technique is a variation on UV shadowing. The technique is based on the observation that some storage phosphor screens (Kodak in this case) are sensitive to UV light in the range where nucleic acids absorb most strongly. Nucleic acid present in a gel resting on the screen absorbs the ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1990
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.56.6.1516-1517.1990